![]() ![]() The proposal of transplanting fabricated BA microtissues, the microtissue fabrication method, and the demonstration of efficacy in T2DM mice are all new. To our best knowledge, this is the first report on fabricating human BA microtissues and showing their safety and efficacy in T2DM mice. Transplanted microtissues impacted multiple organs, secreted protein factors, and influenced the secretion of endogenous adipokines. They alleviated body weight and fat gain and improved glucose tolerance and insulin sensitivity in high-fat diet (HFD)-induced OB and T2DM mice. Fabricated microtissues could survive in vivo for long term. The microtissues could be produced at large scales via 3D suspension assisted with a PEG hydrogel and could be cryopreserved. We found that 3D culture promoted BA differentiation and UCP-1 expression, and the optimal initial cell aggregate size was 100 μm. Here, we reported that preparing BAs as injectable microtissues could overcome the problem. There is a critical need for novel methods that can avoid cell death during cell preparation, transplantation, and in vivo. The enzymatic dissociation during the harvest of fully differentiated BAs also loses significant cells. However, transplanted BAs have a very low survival rate in vivo. Transplanting cell cultured brown adipocytes (BAs) represents a promising approach to prevent and treat obesity (OB) and its associated metabolic disorders, including type 2 diabetes mellitus (T2DM). These hemostatic MNAs may enable rapid hemorrhage control, particularly for patients in developing countries or remote areas with limited or no immediate access to hospitals. Furthermore, the tissue adhesive properties and mechanical interlocking support the suitability of MNAs for wound closure applications. SN-containing MNAs outperform the hemostatic effect of needle-free patches and a commercial hemostat in vivo via combining micro-and nanoengineered features. The engineered MNAs reduce bleeding by ~92% compared with the untreated injury group in a rat liver bleeding model. The SNs render the MNAs hemostatic, while the needle-shaped structure increases the contact area with blood, synergistically accelerating the clotting time from 11.5 min to 1.3 min in vitro. Here, biocompatible, and biodegradable microneedle arrays (MNAs) based on gelatin meth-acryloyl (GelMA) biomaterial hybridized with silicate nanoplatelets (SNs) are developed for hemorrhage control. Developing new biomaterials that are easy to use by non-expert patients and promote rapid blood coagulation is an unmet medical need. If not controlled in a timely manner, exsanguination can take lives within a few minutes. Along with horticultural knowledge, the outcome from the study is expected to provide decision-making information for robotic pollination.īlood loss by hemorrhaging wounds accounts for over one-third of ~5 million trauma fatalities worldwide every year. This information can be used to calculate the percentage of the king flowers and the distribution in the tree canopy. The king flower detection accuracy varied from 98.7% to 65.6% with respect to the flower stages of 20% to 80% blooming. The flower detection accuracy resulting from the algorithm were compared with ground truth measurements. A Mask R-CNN-based detection model followed by a king flower segmentation algorithm were developed to identify and locate the king flowers from an apple flower dataset throughout the blooming stage from first king bloom to full bloom. ![]() In this study, a machine vision system was developed to acquire images for two apple varieties in the orchard environment. Therefore, it is critical to monitor the flower blooming stage for accurately determining the pollination targets and timing. Apple flower clusters open sequentially from the king flower to the lateral flowers in the time of anthesis, which presents an opportunity for selective pollination. King flowers share identical features (e.g., color, shape, and size) with other flowers. Typically, each cluster has five to six individual flowers, and the king flower can be occluded by the lateral flowers because of their central position in a flower cluster. Identification of individual king flowers within flower clusters is a critical step for developing a robotic apple pollination system. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |